The dynamics of biochemical markers of enzymoemia and middle-weight molecules in the early post-traumatic period of brain injury

In the experimental model of brain injury (BI) markers of enzymoemia (aminotransferases ALT and AST, alkaline and acid phosphatase (AlP and AcP), cathepsin D (CD)) and middle-weight molecules (MWM) were estimated. 3 hours after trauma there were increase of all enzymes that evidences about damage of cytoplasmic (ALT, AST, AlF) and lysosomal membranes (AcP, CD). During period of observation different dynamics of enzymatic changes occurred: plateau phase for ALT and CD and progressive increase of concentration with the maximum on the 5th day of the experiment for AlP and AcP. Increase of the level of MWM displayed the development of endogenous intoxication in the post-traumatic period. Relation between particular components of MWM was studied. It was shown that primary accumulation of nucleotide fraction and aromatic peptides is adverse factor of the course of post-traumatic period, development of endotoxicosis, and multiorgan failure.