Screening for ESβL producing E. coli isolated from clinical urine samples collected from different places of Kalaburagi city

Bacteria are capable of invading and infecting humans, leading to disease and sometimes death. Different body tissues, organs and systems are vulnerable to different organisms. This study was aimed to isolate and identify the bacteria causing Urinary Tract Infection (UTI) in different age groups and also screened for ESβL production. The mid-stream urine samples showing symptoms of urinary tract infections were collected in a wide container from different hospitals and diagnostic centres of Kalaburagi city. The isolation of uropathogen was done by semi-quantitative method of inoculating the samples on the selective and differential media such as Eosin Methylene Blue (EMB) and MacConkey (MAC) agar media respectively. The isolated pathogen was identified by conventional methods like cultural, morphological and biochemical tests. The antibiotic susceptibility test was carried out by Kirby-Bauer disc diffusion technique and ESβL production by double disk-diffusion test (DDDT) as per CLSI guidelines (2013). Out of 550 samples screened, a total of 288 bacteria were isolated of those half of them (146) were E. coli isolates and all were found to be multidrug resistant and half of them (50%) were of ESβL producers. All ESβL producing E. coli isolates were resistant to ceftazidime and exhibited higher level of resistance to Cephalothin, Erythromycin, Cotrimoxazole and Aztreonam. As ESβL producing organisms limits the available treatment options, so, the timely administration of sensitive antibiotic and avoiding antibiotic abuse will help in curing the disease without going for the costly drugs such as Carbapenems. Our study revealed that nitrofurantoin, ofloxacin and cefoxitin may be considered as drug of choice for the treatment of UTI patients. The ESβL production in uropathogens should be continuously monitored in the clinics and hospitals as to avoid the emergence of more multi drug resistance strains.