Pomiary liczby poruszających się owadobójczych nicieni przy wykorzystaniu komputerowej analizy obrazu

Przedstawiono badania nad zastosowaniem programu komputerowego do zmierzenia liczby poruszających się w próbach cieczy owadobójczych nicieni. W opracowanym programie komputerowym wykorzystano analizę zmian położenia środków ciężkości obserwowanych obiektów na podstawie serii zdjęć wykonanych za pomocą kamery cyfrowej zamontowanej na mikroskopie. Podczas badań wyznaczono zakres przemieszczenia akceptowalnego środków obiektów, dla którego uzyskano najdokładniejsze wyniki pomiarów.

Results of a study carried out to check a computer program for quantification of entomopathogenic nematodes moving in a liquid sample there are presented in the paper. Nematodes are a component of many biological pesticides used in plant protection, in particular in organic agriculture. In order to define the quality of biopesticides, it is necessary to quantify the live nematodes. Infectious juveniles of entomopathogenic nematodes Steinernema feltiae were the material used in this study. Concentration of the larvae was approximately 140 items in 1 mililiter of liquid. Samples of liquid containing nematodes were photographed in time intervals of 5, 10, 15 and 20 seconds with a digital camera mounted on a microscope. The photographs were subsequently introduced into the computer program prepared in LabView 7.0 by National Instruments Compa-ny. Changes in placements of centers of gravity in the objects between pairs of binary displays were analyzed. The program cal-culated translocation of inertia centers of all objects between their location in first and second photographs. Based on the trials, translocation value and interval time between photographs were selected in order to obtain high accuracy of measurements of moved objects. Calculation results of moved nematodes obtained from the computer program were compared with the results made by researcher's analysis of the same photographs. The results obtained from both methods were identical within the translocation range 1.6 - 4.0 pixels and interval times of 10 and 15 seconds between photographs. Study results indicate the possibility of practical implementation of this developed program for quantification of living nematodes.