Przemiany aniliny w hodowlach bakterii glebowych

During an intensive screening programme, 30 strains of bacteria able to transform of aniline were isolated. The isolated bacteria could transform aniline both under aerobic and anaerobic conditions. On the base of TLC analysis it was possible to observe formation of two main metabolites in culture media of investigated strains of bacteria. These metabolites were named M-l and M-2. It was not possible to isolate the metabolite M-2 from aerobic and anaerobic culture media. The metabolite M-l was isolated from culture medium of strain No 9 growing under aerobic condition. Using Rf value, melting point determination and UV and IR spectra metabolite M-l was identified as a acetanilide. The investigated strain No 9 was classified to generus Bacillus sp. and was the most active in transformation of aniline during growth in Czapek medium at pH 7,0 and incubation temperature 30°C.